@article{23191,
  author = {Minz Mukut and Jha V. and Nada Ritambhra and Mahakur Sobhana and Saikia Biman and Minz Ranjana and Anand Shashi and Sharma Ashish and Joshi Neha and Goel Lekha and Arora Amit and Joshi Kusum},
  title = {Allo-specific immune response profiles indicative of acute rejection in kidney allografts using an in vitro lymphocyte culture-based model.},
  abstract = {<p>
<b>BACKGROUND: </b>Ability to predict the manner in which a recipient's immune system would respond to a transplanted graft by analyzing cytokine profiles of the "allograft antigen sensitized" recipient lymphocytes in vitro might provide a means to identify patients at risk to adverse clinical endpoints.
</p>

<p>
<b>METHODS: </b>Cytokine/chemokine gene expression profiles of peripheral blood mononuclear cells co-cultured with allograft antigen-pulsed macrophages were studied in 49 renal transplant recipients-12 with acute cellular rejection (ACR) with or without antibody-mediated rejection (AMR), 7 with AMR (without ACR), and 30 with stable allografts (SA). An 86-gene inflammatory cytokines and receptors PCR array was used to measure fold changes in gene expression between pulsed and un-pulsed cultures.
</p>

<p>
<b>RESULTS: </b>On linear discriminant analysis and multivariate analysis of variance, a gene set comprising C3, CCL3, IL1B, TOLLIP, IL10, CXCL5, ABCF1, CCR3, IL10RB, CXCL1, and IL1R1 differentiated the ACR-AMR from the SA group. Similarly, a gene set comprising IL10, C3, IL37, IL1B, CCL3, CARD18, and TOLLIP differentiated the AMR from the SA group. No significant difference was found between the ACR-AMR vs AMR groups.
</p>

<p>
<b>CONCLUSION: </b>Distinct post in vitro stimulation cytokine profiles at the time of transplantation thus correlated with the occurrence of post-transplantation rejection episodes which indicated feasibility of this in vitro model to assess the recipient's anti-graft response at an early stage.
</p>
},
  year = {2017},
  journal = {Clin Exp Nephrol},
  month = {182837016111},
  issn = {1437-7799},
  doi = {10.1007/s10157-017-1469-7},
  language = {eng},
}