02240nas a2200241   4500000000100000008004100001260001700042100001500059700001100074700001900085700002000104700001700124700001700141700001700158700001800175700001500193700001500208700001500223700001600238245014400254520158600398022001401984       2017                            d  c1828370161111 aMinz Mukut1 aJha V.1 aNada Ritambhra1 aMahakur Sobhana1 aSaikia Biman1 aMinz Ranjana1 aAnand Shashi1 aSharma Ashish1 aJoshi Neha1 aGoel Lekha1 aArora Amit1 aJoshi Kusum00aAllo-specific immune response profiles indicative of acute rejection in kidney allografts using an in vitro lymphocyte culture-based model.3 a<p>
<b>BACKGROUND: </b>Ability to predict the manner in which a recipient's immune system would respond to a transplanted graft by analyzing cytokine profiles of the "allograft antigen sensitized" recipient lymphocytes in vitro might provide a means to identify patients at risk to adverse clinical endpoints.
</p>

<p>
<b>METHODS: </b>Cytokine/chemokine gene expression profiles of peripheral blood mononuclear cells co-cultured with allograft antigen-pulsed macrophages were studied in 49 renal transplant recipients-12 with acute cellular rejection (ACR) with or without antibody-mediated rejection (AMR), 7 with AMR (without ACR), and 30 with stable allografts (SA). An 86-gene inflammatory cytokines and receptors PCR array was used to measure fold changes in gene expression between pulsed and un-pulsed cultures.
</p>

<p>
<b>RESULTS: </b>On linear discriminant analysis and multivariate analysis of variance, a gene set comprising C3, CCL3, IL1B, TOLLIP, IL10, CXCL5, ABCF1, CCR3, IL10RB, CXCL1, and IL1R1 differentiated the ACR-AMR from the SA group. Similarly, a gene set comprising IL10, C3, IL37, IL1B, CCL3, CARD18, and TOLLIP differentiated the AMR from the SA group. No significant difference was found between the ACR-AMR vs AMR groups.
</p>

<p>
<b>CONCLUSION: </b>Distinct post in vitro stimulation cytokine profiles at the time of transplantation thus correlated with the occurrence of post-transplantation rejection episodes which indicated feasibility of this in vitro model to assess the recipient's anti-graft response at an early stage.
</p>
  a1437-7799